Detection of Sperm DNAAlterations and Heat Shock Protein -70 Levels in Albino Rats Exposed to Methoxychlor

Methoxycholor (C H Cl O ) is an organochorine insecticide used for the control of parasites in 16 15 3 2 livestock as well as a variety of pests on ornamentals, fruits and vegetables. The current investigation threw the light on the possible effect of MXC on the DNA of rat’s spermatozoa using RAPD-PCR technique and the level of HSP-70 of seminal plasma. To achieve this task, 20 adult male rats were used and classified into 4 equal groups. 1 group of rats was gavaged with sesame oil (vehicle) and the remaining 3 groups received st methoxychlor. The second group received 5mg/kg/d for 10 days, the 3 group treated with 50mg/kg/d and the rd 4 group gavaged with 150 mg/kg/d for 10days. To capture spermatozoa first exposed to methoxychlor during th spermiogenesis, animals were sacrificed by decapitation on 21 days after the initiation of treatment. Methoxychlor induced DNA alterations in dose dependent pattern as elucidated by using RAPD-PCR and denrogarmatic analysis. On the similar ground, methoxychlor provoked significant increases in HSP-70 of seminal plasma in dose parallel behavior. It was concluded that methoxychlor induced oxidative stress in epididymal sperm of rats via elevation of HSP 70 of seminal plasma and alterations in DNA fingerprinting.